Web site. The original protocol was changed right after a consultation with all the NordForsk Panel and Scientific Committee members after the trial had been started for the following reasons: (i) a shorter period of intervention (i.e. 18 weeks) was deemed to provide exactly the same information as that?2013 The Association for the Publication of the Journal of Internal Medicine Journal of Internal Medicine, 2013, 274; 52?6M. Uusitupa et al.Healthful Nordic diet plan and CVD riskHealthy nordic diet4 week habitual diet program run-in periodControl diet0 2 4 8 12 (16) 18 (20)?Randomization Adipose tissue biopsy PBMCs Oral glucose tolerance test Biomarkers Anthropometry Biochemistry Blood pressurePBMCs Biomarkers Oral glucose tolerance test Anthropometry Biochemistry Blood stress four day meals diary Ahead of weeks: 0, 2, 12 and 18 orFinal pay a visit to: week 18 or 24 Adipose tissue biopsy PBMCs Oral glucose tolerance test Biomarkers Anthropometry Biochemistry Blood pressureFig. 1 Study design and style inside the Healthy Nordic diet program intervention study. In 4 centres, the key outcome measurements have been taken at week 18. PBMCs = peripheral blood mononuclear cells.obtained from 24 weeks’ trial, (ii) total costs will be lowered, and (iii) recruitment of study subjects was a lot easier for any shorter trial. Hence, it was decided to shorten the intervention to 18 (+/?1) weeks in 4 centres (Aarhus, Uppsala, Reykjavik and Oulu), whereas in Lund and Kuopio exactly where the intervention was started earlier, the original study style was followed. In Kuopio and Lund, the intervention was carried out from October 2009 to June 2010, in Aarhus, from January 2010 to September 2010, in Oulu, from December 2009 to October 2010, in Reykjavik, from March 2010 to October 2010 and in Uppsala, from June 2010 to November 2010. The visits for the study centres have been in line using the original study program, and also the study was strictly blinded regarding the measurements until the trial was completed in all centres. Therefore, in 4 centres with a shorter intervention (18 weeks), the final measurements in line with the original study program had been taken at 18 weeks. The study participants had been advised to help keep weight and physical activity constant and not to transform their smoking and drinking habits or drug therapy for the duration of the study. All study participants offered their written informed consent, and regional Ethical committees of each of the participating centres authorized the study protocol. Screening of study participants and inclusion and exclusion criteria A screening examination was carried out four weeks before the commence with the dietary intervention.1314771-79-3 Chemscene This visit54 ?2013 The Association for the Publication of the Journal of Internal Medicine Journal of Internal Medicine, 2013, 274; 52?integrated healthcare history and a clinical examination, including ECG (if deemed needed by the study physician), body weight, height, waist circumference, blood pressure, blood count, fasting plasma glucose (screening worth 7 mmol L?) and fasting serum creatinine, thyroid-stimulating hormone (TSH), liver enzymes (gamma-glutamyltransferase, alanine aminotransferase, alkaline phosphatase), serum triglycerides, total cholesterol and HDL cholesterol.Buy2,4-Dimethylpyrimidin-5-ol The inclusion criteria were age 30?5 years, BMI 27?38 kg m? and two other of IDF’s criteria for MetS [2], fasting plasma glucose 7.PMID:33597323 0 mmol L? in addition to a 2-h glucose value 11.1 mmol L? at baseline detected by an oral glucose tolerance test. Because the applied IDF criteria [2] concern the white population, only whites have been integrated in t.