Product Name :
PIAS1 Recombinant Rabbit Monoclonal Antibody [JE30-36]
Predicted band size :
68 kDa
Observed band size :
72 kDa
Synonyms:
AR interacting protein antibody DDXBP1 antibody DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody DEAD/H box binding protein 1 antibody DEAD/H box-binding protein 1 antibody E3 SUMO-protein ligase PIAS1 antibody GBP antibody Gu binding protein antibody Gu-binding protein antibody GU/RH-II antibody 展开
Function :
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at’Lys-65′ and ‘Lys-160’. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within Human PIAS1 aa 600-651.5,5′-Oxybis(isobenzofuran-1,3-dione) Chemical name
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IHC-P
Coupling :
unconjugated
Clone number:
JE30-36
RRID:
AB_3072159
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.63649-29-6 uses Aliquot store at -20℃.PMID:33728506 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 68 kDa
Positive control :
MCF-7 cell lysate, HL-60 cell lysate, rat testis tissue lysate, rat testis tissue, human tonsil tissue, human thyroid tissue, human colon carcinoma tissue, mouse hippocampus tissue, mouse brain tissue, NCI-H441.